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[目的]观察生大黄致黑变病结肠Cajal间质细胞(ICC)的细胞结构改变及相关神经递质的变化。[方法]建立豚鼠结肠黑变病模型,观察生大黄致豚鼠黑变病结肠ICC的变化及凋亡情况,并检测结肠组织中相关血管活性肠肽(VIP)、P物质(SP)、一氧化氮(NO)的含量。[结果]黑变病豚鼠结肠ICC细胞胞质中线粒体部分空泡状或双层膜结构消失,内质网扩张,部分内质网表面核糖体脱落;结肠组织细胞凋亡数增加;VIP等神经递质数量差异无统计学意义。[结论]长期应用生大黄等蒽醌类药物可致ICC细胞形态的变化,结肠上皮细胞凋亡增加,其变化不排除与黑变病结肠的排便功能下降相关。
[Objective] To observe the changes of cell structure and related neurotransmitter in Cajal interstitial cells (ICC) of Rhubarb black degeneration. [Method] The model of colonic melanosis in guinea pigs was established. The changes of ICC and the apoptosis of colonic mucosa in guinea pigs induced by rhubarb were observed. The expressions of vasoactive intestinal peptide (VIP), substance P (SP) Nitrogen (NO) content. [Result] Part of vacuolar or bilayer membrane structure of mitochondria disappeared in cytoplasm of ICC cells of guinea pig with melanosis, the endoplasmic reticulum was dilated, part of endoplasmic reticulum surface ribosome shedding; the number of apoptosis in colonic tissue increased; No significant difference in the number of neurotransmitters. [Conclusion] Long-term application of rhubarb and other anthraquinones can change the morphology of ICC cells and increase the apoptosis of colonic epithelial cells. The changes of ICC cells are not related to the decline of defecation function of melanosis colon.