论文部分内容阅读
对水霉（Ｓａｐｒｏｌｅｇｎｉａ）的分离培养技术进行了探索，筛选出大麻籽、油菜籽、红花籽、苍蝇、蚜虫、小麦粒、玉米片、茶叶、葵花仁９种材料作为诱发水霉菌的诱饵。其中油菜籽能防止腐霉菌（Ｐｙｔｈｉ－ｕｍ）污染，是初步纯化菌种、排除杂菌的适宜饵料。同时发现控制温度能使水霉在最短时间内分别产生无性或有性繁殖。在水培条件下，２５℃左右适合菌丝及无性器官的发育，有性器官却不能产生，而只有在１５～１７℃条件下才能使水霉很快进入有性生殖阶段；在加入２００ｍｇ／Ｌ链霉素的ＣＭＡ培养基上促发有性阶段则需要在２５℃左右的温度下进行，方能缩短培养时间。
The technology of isolation and culture of Saprolegnia was explored, and 9 kinds of materials of hemp seeds, rapeseed, safflower, flies, aphids, wheat grains, corn flakes, tea and sunflower kernels were selected as baits for inducing the fungus. Among them, rapeseed can prevent Pythi-um pollution, which is a proper bait for preliminary purification of bacteria and elimination of bacteria. At the same time, it was found that the control temperature can make the water mold produce the asexual or sexual reproduction respectively in the shortest time. Under hydroponics, about 25 ℃ suitable for the development of mycelium and asexual organs, sexual organs can not produce, but only in 15 ~ 17 ℃ conditions to make the mold quickly into the sexual reproductive stage; adding 200mg / L Streptomycin CMA medium to promote the sexual stage you need to be carried out at a temperature of about 25 ℃, in order to shorten the incubation time.