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本文探讨白细胞介素 10 (IL 10 )在小儿原发性肾病综合征 (PNS )中的变化及其与病理形态之间的关系。应用逆转录 多聚酶链反应 (RT PCR )及ELISA法检测了PNS患儿PBMCIL 10mRNA及蛋白水平的变化 ,并分析其与病理形态改变关系。结果 :( 1)PNS患儿急性期IL 10mRNA与蛋白水平较正常对照组显著升高 (P均 <0 0 1) ;而缓解期与正常对照组相比较无显著差异 (P均 >0 0 5 ) ;( 2 )IL 10表达与患儿肾脏病理形态存在一定关系 ,系膜增生性肾炎及IgA肾病明显高于正常对照组 ,微小病变、局灶节段性肾小球硬化和膜增生性肾小球肾炎与正常组比较无显著差异。PBMCIL 10可作为观察病情活动、判断病理类型的一个免疫学指标。IL 10在PNS发病中可能具有一定的抗炎作用。
This article investigates the changes of interleukin 10 (IL 10) in children with primary nephrotic syndrome (PNS) and its relationship with the pathology. The mRNA and protein levels of PBMCIL 10 in children with PNS were detected by reverse transcriptase polymerase chain reaction (RT PCR) and ELISA, and their relationship with pathological changes was analyzed. Results: (1) The levels of IL-10mRNA and protein in acute stage of PNS were significantly higher than those in normal control group (all P <0 01), while those in remission stage were not significantly different from those in normal control group (all P 0 05 ); (2) The expression of IL-10 had some relationship with the pathological changes of kidney in children. The mesangial proliferative glomerulonephritis and IgA nephropathy were significantly higher than those in normal control group, micro-lesion, focal segmental glomerulosclerosis and membranoproliferative kidney Glomerulonephritis compared with the normal group no significant difference. PBMCIL 10 can be used as an immunological indicator to observe the disease activity and determine the pathological type. IL 10 may have some anti-inflammatory effects in the pathogenesis of PNS.