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以水蒸气蒸馏法分别提取吴茱萸五加叶和茎皮的挥发性成分,并采用气相色谱-质谱联用技术(GC-MS)对其进行成分分析。结果从叶中鉴定出28种成分,占叶总挥发性成分的97.31%;从茎皮中鉴定出36种成分,占茎皮总挥发性成分的92.61%。以脂多糖(LPS)刺激RAW 264.7细胞,分别用叶和茎皮的挥发性成分进行干预,测定细胞存活率和一氧化氮(NO)的含量,结果表明在0~20μg/m L浓度范围内两个部位的挥发性成分均表现出轻微的细胞毒性,但叶挥发性成分表现出了显著的一氧化氮抑制活性。本研究为开发利用吴茱萸五加这一丰富的民间药物资源并从中寻找新的天然抗炎物质提供科学基础。
Volatile components of leaves and bark of five leaves of Evodia rutaecarpa were extracted by steam distillation and their components were analyzed by gas chromatography-mass spectrometry (GC-MS). Results From the leaves of 28 components were identified, accounting for 97.31% of the total leaf volatile components; 36 components were identified from the bark, accounting for 92.61% of the total volatile components of the bark. RAW 264.7 cells were stimulated with lipopolysaccharide (LPS), and the cell viability and the content of nitric oxide (NO) were measured by the volatile components of leaves and stem bark respectively. The results showed that in the concentration range of 0 ~ 20μg / ml Volatile components in both sites showed slight cytotoxicity, but leaf volatile components showed significant nitric oxide inhibitory activity. This study provides a scientific basis for the development and utilization of the rich folk medicine resources of Fructus Evodiae and to find new natural anti-inflammatory substances.