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目的:检测染色体8q21~24区域E2F5和MYC癌基因在前列腺癌(prostate cancer,PCa)细胞系、癌组织及癌旁组织中的表达,分析其表达水平与临床病理参数的关系,并探讨其意义。方法:采用DNAqPCR检测PCa细胞系Du145、LNCap、PC3和PCa组织标本中E2F5和MYC基因DNA拷贝数,Western blot和免疫组织化学技术检测前列腺癌和癌旁组织中E2F5和MYC蛋白的表达,结合患者临床病理参数进一步验证和分析蛋白表达及其临床意义。结果:DNA qPCR结果显示,PCa组织中E2F5和MYCDNA拷贝数较癌旁组织明显增加(P<0.05或P<0.01),但在癌细胞系中其表达与对照组比较无明显变化(P>0.05)。Western blot显示,在PCa组织中E2F5和MYC蛋白表达水平显著高于癌旁组织(P<0.05或P<0.01)。免疫组化染色发现,与癌旁良性组织相比,E2F5和MYC蛋白表达显著增加(P均<0.01)。而且,E2F5的过表达与高的Gleason评分(P<0.01)、临床分期(P=0.01)、阳性转移(P<0.01)、生化复发阳性(P<0.01)相关。MYC的过表达与阳性转移(P=0.02)、生化复发阳性(P=0.02)相关。且PCa组织中E2F5和MYC蛋白表达两者呈正相关关系(rs=0.5,P<0.01)。结论:E2F5和MYC的表达增加可能与PCa的发生发展密切相关,E2F5可能是PCa新的潜在候选分子标志物。
OBJECTIVE: To detect the expression of E2F5 and MYC oncogene in prostate cancer (PCa) cell lines, cancer tissues and paracancerous tissues in chromosome 8q21 ~ 24 region, and to analyze the relationship between the expression levels and clinical pathological parameters . Methods: The DNA copy number of E2F5 and MYC in Du145, LNCap, PC3 and PCa were detected by DNAqPCR. The expressions of E2F5 and MYC in prostate cancer and paracancerous tissues were detected by Western blot and immunohistochemistry. Clinicopathological parameters further validate and analyze protein expression and its clinical significance. Results: DNA qPCR showed that the copy number of E2F5 and MYCDNA in PCa tissues was significantly higher than that in paracancer tissues (P <0.05 or P <0.01), but there was no significant difference in the expression of PCNA between the two groups (P> 0.05) ). Western blot showed that the expression of E2F5 and MYC protein in PCa tissues was significantly higher than that in paracancerous tissues (P <0.05 or P <0.01). Immunohistochemical staining showed that the expression of E2F5 and MYC protein were significantly increased (P <0.01) compared with the adjacent normal tissues. Moreover, overexpression of E2F5 correlated with high Gleason score (P <0.01), clinical stage (P = 0.01), positive metastasis (P <0.01) and biochemical recurrence (P <0.01). Overexpression of MYC correlated with positive metastasis (P = 0.02) and biochemical recurrence (P = 0.02). There was a positive correlation between E2F5 and MYC protein expression in PCa (rs = 0.5, P <0.01). Conclusion: The increased expression of E2F5 and MYC may be closely related to the occurrence and development of PCa. E2F5 may be a new potential candidate molecular marker for PCa.