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目的 建立犬吸入全氟异丁烯 (PFIB)致急性呼吸窘迫综合征 (ARDS)模型 ,动态观察演变过程 ,初步探究其损伤机制。方法 自行设计犬染毒装置 ,控制PFIB浓度在 0 .30～ 0 .32mg L ,摸索合适的染毒时间 ,并动态采集血清标本 ,检测白细胞介素 (IL) 6、IL 8含量 ;观察临床表现和器官病理变化。结果 (1)染毒过程中PFIB的浓度稳定 ;(2 )染毒后动物血氧分压逐步下降 ,直至ARDS水平 ;(3)犬血清IL 8水平 [(0 .2 3± 0 .0 11)ng ml]较染毒前 [(0 .12± 0 .0 0 2 )ng ml]明显升高 ,差异有显著性 (P <0 .0 5 ) ,而IL 6水平 [(0 .2 3± 0 .0 4 5 )ng ml]未发现明显改变 ,较染毒前 [(0 .2 2± 0 .0 0 6 )ng ml]的差异无显著性 (P >0 .0 5 ) ;(4 )染毒后 6h内犬无异常表现 ,其后症状逐渐出现 ,后期表现为典型的ARDS频速浅快呼吸症状 ;(5 )病理观察发现犬双肺绝大部分有严重充血、水肿和不张 ,其他脏器改变为器官缺氧的表现。结论 (1)所设计的犬染毒装置实现了染毒可控性 ;(2 )染毒 30min ,犬 2 2h后均达ARDS临床诊断标准 ,模型建立方法成功 ;(3)PFIB特异性损伤肺 ,可引起肺的过度炎症反应。
Objective To establish a model of acute respiratory distress syndrome (ARDS) induced by inhalation of perfluoroisobutylene (PFIB) in dogs and observe its evolution dynamically to explore its mechanism of injury. Methods To design a dog-toothed device to control the concentration of PFIB at 0.30-0.32mg L, explore the appropriate exposure time, and serum samples were collected dynamically to detect the content of interleukin (IL) 6 and IL-8; to observe the clinical manifestations And organ pathological changes. Results (1) The concentration of PFIB in the course of exposure was stable. (2) The partial pressure of oxygen decreased gradually until the level of ARDS after exposure. (3) The level of IL-8 in the serum [(0.23 ± 0.10 11 ) ng ml] was significantly higher than that before exposure [(0.12 ± 0 .002) ng ml], the difference was significant (P <0.05), while the level of IL-6 [(0.23 There was no significant difference between the two groups (P> 0.05). There was no significant difference between the two groups (P> 0.05) 4) The dogs showed no abnormalities within 6h after exposure, and the symptoms gradually appeared later, with the typical ARDS frequency of fast breathing symptoms. (5) Most of the canine lungs were found to have severe hyperemia, edema and no Zhang, other organs changed to the performance of organ hypoxia. Conclusions (1) The designed canine-toothed device achieves the controllability of exposure. (2) The clinical diagnostic criteria of ARDS reaches to the standard of ARDS after being exposed to the drug for 30 minutes and the model is successfully established. (3) , Can cause excessive inflammation of the lungs.