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用中间偃麦草2Ai-2染色体特异的EST-PCR标记检测5个小麦-中间偃麦草二体异代换系(包括端体代换系)与普通小麦中国春(CS)杂交后代群体,研究外源染色体2Ai-2通过杂种向后代的传递率及其结构变异,并用基因组原位杂交进行验证。结果表明,第二部分同源群不同染色体代换背景对外源染色体传递的影响不同,在2B代换系的杂种中外源染色体或片段显示优先传递,而在2D代换系的杂种中其传递力则较低,2B代换背景更有利于2Ai-2染色体或片段的传递;外源染色体在杂种后代传递过程中会发生变异,在多数组合中,变异出现在着丝粒处;与短臂相比,外源染色体长臂更容易在世代中丢失;端体代换系中的外源染色体端体在杂种后代传递过程中容易丢失,且也会发生结构变异。基因组原位杂交结果证明了分子标记跟踪外源染色体的可靠性。
Thinopyrum intermedium (Phytophthora meiiana) 2Ai-2 chromosome-specific EST-PCR markers were used to detect 5 progenies of wheat-Thinopyrum intermedium alien substitution lines (including terminal substitution lines) and common wheat Chinese Spring (CS) The translocation rate and its structural variation of the source chromosome 2Ai-2 to offspring through hybrids were verified by genomic in situ hybridization. The results showed that the second part of the homologous group different chromosomal substitution background on the impact of exogenous chromosome transfer in the 2B substitution lines of hybrids in the foreign chromosomes or fragments showed preferential transmission in the 2D substitution lines of hybrids in its transmission Then the 2B substitution background is more conducive to the transmission of 2Ai-2 chromosomes or fragments. Exogenous chromosomes will mutate during the transmission of hybrid offspring. In most combinations, the variation occurs at the centromere; The long arm of the exogenous chromosome is more likely to be lost in the generation. The exogenous chromosome body in the exon substitution line is easily lost during the transfer of the hybrid offspring, and structural variation also occurs. Genomic in situ hybridization results demonstrate that molecular markers track the reliability of exogenous chromosomes.