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目的以秀丽隐杆线虫为模式生物研究珠蛋白(globin)13(glb-13)的生理作用,以回交2次的glb-13(tm2825)突变株系和hif-1(ia04)构建线虫双突变株系hif-1(ia04);glb-13(tm2825)。方法 glb-13(tm2825)与野生型N2株系交配获得glb-13(tm2825)的雄虫,再与N2的雌雄同体线虫进行交配,回交2次后,通过单只线虫基因组PCR法鉴定出交配后的纯合体。使用hif-1(ia04)和野生型线虫N2进行交配得到hif-1(ia04)的雄虫,然后再与glb-13(tm2825)雌雄同体线虫交配,通过单只线虫基因组PCR法鉴定出交配后的纯合体。结果获得hif-1(ia04);glb-13(tm2825)双突变株系。结论通过线虫交配回交可以使突变株系线虫保持稳定性状,而突变株系之间的交配可以制备出双突变株系,为深入开展glb-13的功能研究奠定了物质基础。
OBJECTIVE: To investigate the physiological role of glb-13 in C. elegans as a model organism and to construct a nematode-double mutant of glb-13 (tm2825) and hif-1 (ia04) Mutant lines hif-1 (ia04); glb-13 (tm2825). Methods The glb-13 (tm2825) was mated with the wild-type N2 strain to get the male of glb-13 (tm2825), and then crossed with the hermaphrodite nematodes of N2. After backcrossing twice, the mating After the homozygotes. Male mice of hif-1 (ia04) were mated with hif-1 (ia04) and wild-type nematode N2 and then mated with the hermaphroditic nematodes of glb-13 (tm2825) Homozygous. The results obtained hif-1 (ia04); glb-13 (tm2825) double mutant lines. CONCLUSION: The nematodes of the mutant strains can maintain the stable trait by mating and backcrossing the nematode, while the double mutant strains can be prepared through the mating of the mutant strains, which lays the material foundation for further study on the function of glb-13.