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目的 :探讨Aβ4 2 及其亚单位肽疫苗接种小鼠后特异性抗Aβ4 2抗体的产生情况。方法 :75只 6wk龄雄性BALB/c小鼠 ,随机分为 5组 :即对照组、Aβ4 2 组 ,Aβ3 6 - 42 组、Aβ1- 15 组和FAβ1- 15组。分别用PBS +MF59佐剂 ,Aβ4 2 +MF59,Aβ36～ 4 2 +七聚赖氨酸 (MAP) +MF59,Aβ1- 15 +MAP +MF59,Aβ1- 15 +MAP +福氏佐剂 ,免疫BALB/c小鼠 4次。用间接ELISA法 ,检测各组免疫小鼠血清和脑组织匀浆上清液中特异性抗体的滴度。将Aβ42 、Aβ3 6 - 42 和Aβ1- 15 与培养的PC12细胞共同培养 7d ,用MTT比色法检测 3种抗原肽对PC12细胞的毒性。将各组免疫小鼠的血清加入到含 2 0mg/LAβ4 2 的培养基中 ,再与培养的PC12细胞一起培养 7d ,用MTT比色法测定PC12细胞的存活率。结果 :第 2次免疫后 ,各实验组小鼠的免疫血清均有抗Aβ42 抗体产生 ,且抗体滴度随接种次数的增多而增高。同时 ,在脑组织匀浆上清液中也可检测出低滴度的抗Aβ4 2 抗体。Aβ42 可降低PC12细胞的存活率 ;而不同浓度的Aβ3 6 - 42 和Aβ1- 15 则对PC12细胞的存活率无显著影响。将 4组疫苗免疫血清和 2 0mg/LAβ42 同时加入培养基中培养PC12细胞时 ,可显著提高其存活率。结论 :Aβ42 及其亚单位疫苗 (Aβ1- 15 及Aβ36～ 4 2 )结合MF59佐剂免疫B
Objective: To investigate the production of specific anti-Aβ4 2 antibody in mice immunized with Aβ4 2 and its subunit peptide. Methods: Seventy-five male BALB / c mice of 6wk age were randomly divided into 5 groups: control group, Aβ4 2 group, Aβ3 6 - 42 group, Aβ1-15 group and FAβ1-15 group. BALB / c mice were immunized with PBS + MF59 adjuvant, Aβ4 2 + MF59, Aβ36-4 2 + heptapoly lysine (MAP) + MF59, Aβ1-15 + MAP59, A59 + Aβ1-15 + MAP + / c mice 4 times. Indirect ELISA was used to detect the titer of specific antibodies in the serum and brain homogenate supernatant of the immunized mice. Aβ42, Aβ3 6 - 42 and Aβ1-15 were cultured with cultured PC12 cells for 7 days. The cytotoxicity of three kinds of antigenic peptides on PC12 cells was detected by MTT assay. The serum of the immunized mice in each group was added to the medium containing 20mg / LAβ4 2, and cultured with the cultured PC12 cells for 7 days. The survival rate of PC12 cells was determined by MTT colorimetry. Results: After the second immunization, the immune sera of mice in each experimental group had anti-Aβ42 antibody production, and the antibody titer increased with the increase of inoculation frequency. At the same time, low titers of anti-Aβ4 2 antibodies were also detected in brain tissue homogenate supernatants. Aβ42 can reduce the survival rate of PC12 cells; while different concentrations of Aβ3 6 - 42 and Aβ1-15 have no significant effect on the survival rate of PC12 cells. Four groups of vaccine immune serum and 20mg / LAβ42 added to the culture medium when cultured PC12 cells, can significantly increase its survival rate. CONCLUSION: Aβ42 and its subunit vaccines (Aβ1-15 and Aβ36-4 2) combined with MF59 adjuvant to immunize B