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研究了河南省著名中药材怀山药和地黄的有效成分,并考察了怀山药和地黄配伍成“药对”对其成分和含量的影响.建立了同时测定不同配比(3∶1、2∶1、1∶1、1∶2、1∶3、1∶5)的怀山药和地黄药对及其单味药材中梓醇、5-羟甲基-2-呋喃甲醛(5-HMF)和腺苷的高效液相色谱法.采用苯酚-硫酸法对不同配比的“药对”中多糖含量进行了定量测定.用Folin-Ciocalteu比色法测定“药对”中多酚类物质的含量.结果表明,“药对”的配伍能提高梓醇的溶出速率.但怀山药和熟地黄的配伍对5-HMF的溶出度有抑制作用.“药对”中腺苷含量随药对比例的变化无明显变化.紫外和可见分光光度法对“药对”及其单味药中多糖和多酚的含量测定结果表明,“药对”中多糖含量与理论计算结果相比随不同比例明显增加.然而,与理论值相比,“药对”中多酚含量没有明显变化.但是复配之后会有一点提高.本研究为中药提取物或中药配方等复杂体系中主要成分的快速测定提供了一个很好的范例,为今后的活性研究和临床应用研究奠定了良好的基础.“,”The active ingredients of Dioscorea opposita Thunb and Rehmannia glutinosa as famous geoherbs in Henan province of China were researched,focusing on the influence of the component and content caused by the compatibility of the two species by forming herb pairs.The high-performance liquid chromatography (HPLC) method for the simultaneous determination of catalpol,5-hydroxymethyl-2-furaldehyde (5-HMF) and adenosine in the medicine pair of Dioscorea opposita Thunb and Rehmannia glutinosa with different proportions (3 ∶ 1,2 ∶ 1,1 ∶ 1,1 ∶ 2,1 ∶ 3,1 ∶ 5)and their single herb medicine was developed.The quantitative determination of polysaccharides content in the medicine pair with different proportions was conducted by using the phenol-sulfuric acid method,and the polyphenolics content in the medicine pair was assayed by using the Folin-Ciocalteu colorimetric method.The results show that the compatibilities of the herb pair can promote the dissolution rate of catalpol.But the compatibility of Dioscorea opposita Thunb and Radix Rehmannia Preparata has an inhibitory action to the dissolution rate of 5-HMF.The content of adenosine in the medicine pair has no obvious change with the variation of the proportions of medicine pair.The contents of polysaccharides and polyphenols in the medicine pair and their single herb medicine conducted by ultraviolet and visible spectrophotometry method indicate that the polysaccharides content in their herb pairs has an obvious increase in different proportions with respect to the theoretically calculated results.However,compared with the theoretical values,the polyphenols content in their herb pairs has no obvious change,showing a slight increase after compatibility.This study provides a good example for the rapid determination of major constituents in complex systems such as herbal extract or traditional Chinese medicine formula,and lays a good foundation for the activity study and clinical application in the future research.