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将200μl血清移至4ml 离心管中,加入200μl生育酚乙酸酯甲醇溶液作为内标(650mg/L),在涡流振荡器中混合30s,加入200μl正己烷,用聚乙烯塞密封振摇15min,离心(3000r/min)10 min,将120μl正己烷提取液移到1.5ml 微离心管中,在 N_2气流下蒸发,
200 μl of serum was transferred to a 4 ml centrifuge tube, 200 μl of tocopherol acetate methanol solution was added as an internal standard (650 mg / L), mixed for 30 s in a vortex shaker, 200 μl of n-hexane was added, the vial was sealed and shaken with a polyethylene plug for 15 min, Centrifugal (3000r / min) 10min, 120μl n-hexane extract was moved to 1.5ml microcentrifuge tube, evaporated under N 2 gas flow,