论文部分内容阅读
目的：探讨载脂蛋白Ｅ（ＡｐｏＥ） 在正常和病变肝组织中的免疫定位分布。方法：应用免疫组化ＳＰ法分别检测正常和病变肝组织ＡｐｏＥ的表达，正常肝组织１２ 例（ 正常肝组织３ 例，癌旁正常肝组织９ 例） ，病毒性肝炎６ 例，肝硬化２２ 例，肝癌３０ 例（Ⅰ级Ⅱ级１３ 例，Ⅲ级Ⅳ级１７ 例） ，肝母细胞癌６ 例，肝海绵状血管瘤４ 例。结果：ＡｐｏＥ 在正常肝细胞广泛表达，为胞浆表达，在临近肝窦一面的肝细胞为胞膜表达，Ｋｕｐｆｆｅｒ 细胞表达阳性，肝内胆管上皮和肝血管内皮细胞不表达；在肝炎、肝硬化、分化较好的肝癌（Ⅰ、Ⅱ级） 表达，在分化较差的肝癌、肝母细胞瘤不表达。结论：ＡｐｏＥ对良性和恶性肝肿瘤的鉴别诊断有帮助
Objective: To investigate the distribution of apolipoprotein E (ApoE) in normal and diseased liver tissues. Methods: The expression of ApoE in normal and diseased liver tissues was detected by immunohistochemical SP method. Twelve normal liver tissues (3 normal liver tissues and 9 adjacent normal liver tissues), 6 viral hepatitis and 22 cirrhosis , 30 cases of hepatocellular carcinoma (Grade Ⅰ Ⅱ 13 cases, Grade Ⅲ Ⅳ 17 cases), hepatocellular carcinoma 6 cases, 4 cases of hepatic cavernous hemangioma. Results: ApoE was widely expressed in normal hepatocytes and expressed in the cytoplasm. The expression of ApoE in the hepatocytes adjacent to the sinusoidal sinusoid was positive, and Kupffer cells were positive. The expressions of ApoE were not expressed in the intrahepatic bile duct epithelium and hepatic vascular endothelial cells. , Well-differentiated liver cancer (Ⅰ, Ⅱ grade) expression, poorly differentiated liver cancer, hepatoblastoma does not express. Conclusion: ApoE is helpful in the differential diagnosis of benign and malignant liver tumors