精原细胞分离纯化方法的建立及其免疫化学鉴定

来源 :中华实验外科杂志 | 被引量 : 0次 | 上传用户:yangmx198808
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读

我们通过程序性复合酶消化结合差速贴壁及非连续性Percoll密度梯度离心的方法纯化精原细胞,探讨精原细胞的分离纯化方法,并对纯化后细胞进行免疫化学鉴定。

其他文献
Facial beauty prediction belongs to an emerging field of human perception nature and rule.Compared with other facial analysis tasks,this task has shown its challenges in pattern recognition and biomet
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生、测量监控等方面人手,介绍了S226海滨大桥
期刊
Binary sequences with large linear complexity have been found many applications in communication systems.We determine the linear complexity of a family of p~2-p
Real-time parameter identification of sinusoidal signals is an essential research topic due to its broad utilization in both theoretical studies and engineering
缝隙连接(GJ)是相邻细胞间跨膜离子及低分子通道[1],逼尿肌缝隙连接介导细胞间通讯(GJIC)功能在DI发病机制中具有重要意义.本研究旨在利用激光扫描共聚焦显微镜(LSCM)及荧光漂白后恢复技术(FRAP)观察缝隙连接阻滞剂18β-GA对培养大鼠DI细胞GJIC功能的影响,为采用阻断细胞间兴奋传递来治疗DI提供依据。
To decrease the overlarge calculation induced by the centralized processing,a new cooperative distributed Model predictive control(MPC)method is proposed for la
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生、测量监控等方面人手,介绍了S226海滨大桥
期刊
With the growing trend of deploying Software defined networks(SDN) in Internet and data centers,it is necessary to investigate the operation of multicast servic