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本文采用高校液相测定了氯地乳膏中氯霉素和醋酸地塞米松的含量,固定相为:安捷伦Cl8(4.6mm×250mm,5μm),甲醇-乙腈-0.025mol/L磷酸二氢钠(25:25:50)为流动相,检测波长为240nm;醋酸地塞米松在10~100μg·m L-1范围内呈良好的线性关系;氯霉素在50~500μg·m L-1范围内呈良好的线性关系。醋酸地塞米松和氯霉素的平均回收率分别为99.5%、99.5%,RSD为0.86%(n=6)、0.83%(n=6);此方法简便、准确、重现性好,可用于氯地乳膏中醋酸地塞米松和氯霉素的含量测定。
In this paper, the content of chloramphenicol and dexamethasone acetate in chlorpheniramine cream was determined by HPLC in the university. The stationary phase was Agilent Cl8 (4.6 mm × 250 mm, 5 μm), methanol-acetonitrile-0.025 mol / L sodium dihydrogen phosphate (25:25:50) as the mobile phase, the detection wavelength was 240nm. Dexamethasone acetate showed a good linearity in the range of 10 ~ 100μg · m L-1. The range of chloramphenicol in the range of 50 ~ 500μg · m L-1 Within a good linear relationship. The average recoveries of dexamethasone acetate and chloramphenicol were 99.5% and 99.5%, respectively. The RSD was 0.86% (n = 6) and 0.83% (n = 6) respectively. The method was simple, accurate and reproducible Determination of Dexamethasone acetate and Chloramphenicol in Chloride cream.