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目的:研究新核糖体失活蛋白-栝楼蛋白是否具有抗HIV-1活性。方法:合胞体抑制实验以光镜检查,以ELISA测定HIV-1 p24抗原表达水平,用间接免疫荧光技术测定HIV抗原阳性细胞的百分率。结果:栝楼蛋白对HIV-1诱导C8166细胞形成合胞体有显著的抑制作用。栝楼蛋白显著地抑制了HIV-1急性感染T细胞中p24核心抗原的表达水平和减少了HIV抗原阳性细胞的百分率。栝楼蛋白抑制合胞体形成和HIV抗原阳性细胞的IC_(50)分别是5μg·L~(-1)和0.09mg·L~(-1)。栝楼蛋白不影响慢性感染T细胞中HIV-1 p24抗原表达水平。结论:栝楼蛋白是具有抗HIV-1活性的新的核糖体失活蛋白。
OBJECTIVE: To investigate whether the new ribosome inactivating protein, Tui Lou protein, has anti-HIV-1 activity. Methods: The inhibition of syncytia was examined by light microscopy. The expression of HIV-1 p24 antigen was detected by ELISA. The percentage of HIV antigen-positive cells was determined by indirect immunofluorescence assay. Results: Tui Lou protein had a significant inhibitory effect on the formation of syncytia of HIV-1-induced C8166 cells. Bream protein significantly inhibited the expression of p24 core antigen in HIV-1 acutely infected T cells and decreased the percentage of HIV antigen-positive cells. The IC 50 of inhibitory syncytia formation and HIV antigen-positive cells in Bream protein were 5 μg · L -1 and 0.09 mg · L -1, respectively. Tui Lou protein does not affect the expression of HIV-1 p24 antigen in chronic infected T cells. CONCLUSION: Bream protein is a new ribosome-inactivating protein with anti-HIV-1 activity.