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由致病疫霉菌(Phytophthora infestans)引起的晩疫病是世界范围内最具毁灭性的马铃薯(Solanum tuberosum)病害。以含有晚疫病抗性基因R11的材料MaR11和不含已知抗性基因的品种Katahdin为亲本进行有性杂交,对获得的F1分离群体的83个基因型进行了晚疫病菌株接种鉴定和遗传分析。结果表明,R11为主效单基因,在MaR11中以单式形式(R11r11r11r11)存在。应用比较作图和分离群体分组分析(BSA),开发了6个与R11连锁的分子标记,并将R11定位于11号染色体长臂末端。R11距C2_At5g59960标记最近,约为2.4 cM。通过遗传图谱比较表明,R11较晚疫病抗性基因R3a和R10更靠近染色体端粒区。本研究所获得的遗传图谱为进一步构建R11高密度遗传图谱提供了基础。
Ruddy blight caused by Phytophthora infestans is the most devastating disease of Solanum tuberosum in the world. Eighty-three genotypes of F1 isolates were inoculated with P. infestans and their genetic analysis was carried out by sexually crossing MaR11 containing the late blight resistance gene R11 and Katahdin without the known resistance gene . The results showed that R11 is the major single gene and exists in MaR11 as a monomeric form (R11r11r11r11). Six molecular markers linked to R11 were developed and mapped to the long arm end of chromosome 11 using comparative mapping and population segregation analysis (BSA). R11 is closest to the C2_At5g59960 marker and is about 2.4 cM. Comparison of genetic map showed that R11 was closer to chromosome telomere than the late blight resistance genes R3a and R10. The genetic map obtained in this study provided the basis for further construction of R11 high-density genetic map.