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目的 :探讨健脾理气方对 HAC肝癌细胞凋亡及 bax基因蛋白表达的影响。方法 :建立小鼠 HAC肝癌荷瘤模型 ,随机分 3组 ,称瘤重 ,计算抑瘤率。用 TU NEL法和电镜检测肿瘤细胞凋亡 ,用免疫组化法检测 bax基因蛋白表达水平。结果 :中药组和化疗组瘤重均低于对照组 (P <0 .0 1) ,抑瘤率分别为 41.6 %和 43.0 %。其凋亡指数较对照组明显升高 (P <0 .0 1)。电镜检测发现凋亡细胞发生特征性形态学改变。对照组 bax基因蛋白无强阳性表达 ,中药组和化疗组均强阳性。结论 :健脾理气方能诱导 HAC肝癌细胞凋亡并上调肿瘤细胞 bax基因蛋白表达 ,可能是该方抗癌机制之一。
Objective : To investigate the effect of Jianpi Liqi Recipe on apoptosis of hepatocellular carcinoma cells and expression of bax gene protein. METHODS: A mouse model of HAC liver cancer was established and randomly divided into 3 groups. The tumor weight was calculated and the tumor inhibition rate was calculated. Tumor cell apoptosis was detected by TUNEL and electron microscope, and the expression of bax gene protein was detected by immunohistochemistry. Results: The tumor weights in the Chinese medicine group and the chemotherapy group were lower than those in the control group (P < 0.01). The tumor inhibition rates were 41.6% and 43.0%, respectively. The apoptotic index was significantly higher than that of the control group (P < 0.01). Electron microscopy revealed characteristic morphological changes in apoptotic cells. In the control group, there was no strong positive expression of bax gene protein, and both the Chinese medicine group and the chemotherapy group were strongly positive. Conclusion :Jianpi Liqi can induce apoptosis of HAC hepatoma cells and upregulate the expression of bax gene protein in tumor cells, which may be one of the anti-cancer mechanisms of this side.