论文部分内容阅读
泡沫细胞的出现是动脉粥样硬化斑块中有特征性的病理形态改变。本实验选择对动脉粥样硬化形成较敏感的纯系小鼠Ｃ５７ＢＬ／６Ｊ，取其腹膜巨噬细胞与１０ｍｇ·Ｌ－１氧化低密度脂蛋白共同孵育９６ｈ，建立了典型的巨噬细胞源性泡沫细胞模型。实验结果显示，培养的泡沫样细胞与在无脂蛋白培养基中培养的Ｃ５７ＢＬ／６Ｊ小鼠巨噬细胞和在氧化低密度脂蛋白中培养的昆明种小鼠巨噬细胞比较，脂质成分大量增多，出现脂质颗粒，细胞内总胆固醇增加，其中胆固醇酯含量大于游离胆固醇，符合泡沫细胞的定义。本实验从离休细胞培养方面探寻建立小鼠巨噬细胞源性泡沫细胞的可能性，为动脉粥样硬化的形成机理分析和防治研究提供了一种可靠的病理细胞模型。
The appearance of foam cells is a characteristic pathological change in atherosclerotic plaque. In this study, C57BL / 6J, a pure mouse model of atherosclerosis, was selected and co-incubated with peritoneal macrophages and 10mg · L-1 oxidized low density lipoprotein for 96h to establish a typical macrophage-derived Foam cell model. The experimental results showed that compared with the macrophages of C57BL / 6J mice cultured in lipoprotein-free medium and the Kunming mouse macrophages cultured in oxidized low-density lipoprotein, the number of lipid components Increased, there are lipid particles, intracellular total cholesterol increased cholesterol ester content greater than free cholesterol, in line with the definition of foam cells. In this study, we explored the possibility of establishing mouse macrophage-derived foam cells from the culture of retired cells and provided a reliable pathological cell model for the analysis of the formation mechanism and prevention and treatment of atherosclerosis.