论文部分内容阅读
目的检测脑缺血再灌注(I/R)后海马齿状回区(dentate gyrus,DG)星形胶质细胞活化增殖及其磷酸化信号转导与转录激活子3(phosphorylated STAT3,p-STAT3)表达情况,为调控星形胶质细胞异常活化增殖提供理论依据。方法线栓法制作大鼠脑局部I/R模型,48只Sprague-Dawley(SD)大鼠随机分为假手术(Sham)组、I/R-24 h组、I/R-72 h组及I/R-7 d组。免疫荧光法及Western印迹检测缺血后不同时间点患侧海马区胶质原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)标记阳性细胞及p-STAT3表达情况。结果与Sham组比较,I/R-72 h和I/R-7 d组海马区GFAP蛋白表达增加(P<0.05);随缺血时间延长,GFAP蛋白表达增加呈上升趋势,且24 h、72 h和7 d各时间点之间两两比较差异均具有统计学意义(P<0.05)。与Sham组比较,脑I/R后海马区各时间点p-STAT3表达量均显著增加(P<0.05);p-STAT3表达在24 h最高,72 h开始下降,7 d仍有一定量表达,与I/R-24 h组比较,I/R-72 h组和I/R-7 d组p-STAT3表达量均显著降低(P<0.05)。结论脑I/R损伤后,海马区星形胶质细胞大量增殖及其p-STAT3表达增加;且p-STAT3的表达高峰在时间上早于星形胶质细胞的大量增殖。
Objective To detect the activation and proliferation of rat hippocampal dentate gyrus (DG) astrocytes and the expression of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) after cerebral ischemia-reperfusion (I / R) ) Expression, to provide a theoretical basis for the regulation of abnormal activation and proliferation of astrocytes. Methods The rat brain I / R model was made by thread method. Forty-eight Sprague-Dawley rats were randomly divided into sham group, I / R-24 h group, I / R-72 h group I / R-7 d group. Immunofluorescence and Western blotting were used to detect the expression of glial fibrillary acidic protein (GFAP) positive cells and p-STAT3 in hippocampus at different time points after ischemia. Results Compared with Sham group, the GFAP protein expression in hippocampus of I / R-72 h group and I / R-7 d group increased (P <0.05). With the prolongation of ischemia, GFAP protein expression increased, The difference between every two time points was statistically significant at 72 h and 7 d (P <0.05). Compared with Sham group, the expression of p-STAT3 in hippocampus of I / R group was significantly increased at each time point (P <0.05). The expression of p-STAT3 was highest at 24 h and then decreased at 72 h, Compared with I / R-24 h group, the expression of p-STAT3 in I / R-72 h group and I / R-7 d group decreased significantly (P <0.05). CONCLUSION: After I / R injury, the proliferation of hippocampal astrocytes and the expression of p-STAT3 increased. The peak of p-STAT3 expression was earlier than that of astrocytes.