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目的探讨宫颈浸润性鳞状细胞癌(ISCC)中β-连结蛋白(β-cat)、细胞周期素D1(CyclinD1)、Ki67及高危型人乳头瘤病毒(HPV)的表达,分析它们在宫颈鳞癌发生、发展中的作用。方法将73例ISCC、30例高级别鳞状上皮内病变(HSIL)、32例低级别鳞状上皮内病变(LSIL)和21份正常宫颈组织(NCE)应用组织微阵列法制成组织芯片,同时采用原位杂交法检测HPV16/18,31/33DNA的表达;免疫组织化学法检测β-cat、Cy-clinD1和Ki67蛋白的表达。采用χ2检验。结果①HPV16/18,31/33在宫颈病变中的阳性表达差异有统计学意义(P<0.01);宫颈鳞癌中HPV16/18阳性患者易发生脉管癌栓(OR=3.875),HPV31/33阳性患者临床期别更易进展为高期别(OR=3.5)。②β-cat的异常表达、CyclinD1和Ki67的阳性表达在宫颈病变中差异有统计学意义(P<0.01);β-cat在LSIL、HSIL和ISCC中为异质性表达。③宫颈鳞癌年龄增大则病理分期增加(OR=1.063)。结论 HPV16/18,31/33的感染与宫颈鳞癌的发生密切相关,并与进展有关;Ki67可作为宫颈病变诊断的客观参考指标。
Objective To investigate the expression of β-cat, CyclinD1, Ki67 and high-risk human papillomavirus (HPV) in cervical invasive squamous cell carcinoma (ISCC) The occurrence of cancer, the role of development. Methods 73 cases of ISCC, 30 cases of high grade squamous intraepithelial lesion (HSIL), 32 cases of low grade squamous intraepithelial lesion (LSIL) and 21 cases of normal cervical tissue (NCE) were made tissue microarray method, at the same time The expression of HPV16 / 18 and 31/33 DNA was detected by in situ hybridization and the expressions of β-cat, Cy-clinD1 and Ki67 were detected by immunohistochemistry. Using χ2 test. Results ① The positive expression rates of HPV16 / 18 and 31/33 in cervical lesions were statistically significant (P <0.01). Vascular tumor thrombus (OR = 3.875) and HPV31 / 33 Positive patients were more likely to progress to a higher clinical stage (OR = 3.5). ② The abnormal expression of β-cat, the positive expression of CyclinD1 and Ki67 in cervical lesions showed significant difference (P <0.01); β-cat was heterogeneous expressed in LSIL, HSIL and ISCC. ③ cervical squamous cell carcinoma increased the pathological stage (OR = 1.063). Conclusion The infection of HPV16 / 18 and 31/33 is closely related to the occurrence of cervical squamous cell carcinoma and is related to the progress of the disease. Ki67 can be used as an objective reference for the diagnosis of cervical lesions.