论文部分内容阅读
目的:应用膜片钳技术记录小鼠耳蜗螺旋神经节细胞的全细胞电流,了解电压依赖性离子通道的基本电生理学特性,并比较耳蜗顶、底转螺旋神经节细胞电生理学特性的差异。方法:应用全细胞构型电压钳制技术,采用不同的电极内液及阻断剂,在不同的刺激参数下记录耳蜗顶、底转螺旋神经节细胞的电压依赖性离子通道电流,并进行分析比较。结果:实验记录到了内向的钠电流、延迟整流钾电流、超极化激活内向阳离子通道电流及瞬时外向钾电流,并发现耳蜗顶、底转螺旋神经节细胞的延迟整流钾电流及瞬时外向钾电流的电生理学特性具有显著性差异(P<0.05)。结论:实验记录到的各种离子电流数据表明耳蜗螺旋神经节细胞具有完成动作电位的形成、传导并对其功能进行调节的离子通道基础;耳蜗顶、底转螺旋神经节细胞电生理学特性的差异有助于听觉的形成过程。
PURPOSE: To record the whole-cell currents of mouse spiral ganglion cells using patch-clamp technique to understand the basic electrophysiological characteristics of voltage-dependent ion channels and to compare the electrophysiological characteristics of cochlear apical and basal ganglion cells. Methods: Whole-cell configuration voltage clamp technique was used to record voltage-dependent ion channel currents of cochlear apices and basal ganglia spiral cells under different stimulation parameters using different electrode fluids and blockers. . Results: Inward sodium current, delayed rectifier potassium current, hyperpolarization-activated inward cation channel current and transient outward potassium current were recorded and the delayed rectifier potassium current and transient outward potassium current were found in the cochlear apical and basal ganglia. Electrophysiological characteristics have significant differences (P <0.05). CONCLUSIONS: Various ion current data recorded in the experiment indicate that the cochlear spiral ganglion cells have the basis of ion channels for the formation, conduction and regulation of action potentials. The electrophysiological characteristics of the cochlear spiral ganglion cells are different Contribute to the formation of hearing.