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目的研究Cr(VI)对果蝇S2细胞增殖及氧化应激相关指标的影响,初步探讨二者的关系,为利用果蝇S2细胞研究Cr(VI)的毒性作用提供一定的理论依据。方法利用含不同浓度Cr(VI)(50、100、200、400、800μmol/L)的培养基培养果蝇S2细胞;CCK-8法检测培养基中不同浓度Cr(VI)对果蝇S2细胞增殖的影响;分光光度法检测培养基上清中SOD、MDA含量。结果随着Cr(VI)浓度升高,S2细胞的生存率逐渐降低并存在显著的剂量依赖关系,当800μmol/L时Cr(VI)对S2细胞的抑制率已高达(88.51±0.72)%;SOD活力自200μmol/L处理浓度开始即与对照组相比差异有统计学意义(P<0.05),随Cr(VI)处理浓度增加SOD含量逐渐降低;各处理组脂质氧化终产物MDA含量与对照相比差异均有统计学意义(P<0.05),随Cr(VI)处理浓度增加MDA含量逐渐升高。结论 Cr(VI)可显著抑制S2细胞增殖,且呈高度剂量依赖性;Cr(VI)可导致S2细胞氧化应激,氧化应激可能是Cr(VI)抑制S2细胞增殖的重要原因之一。
Objective To investigate the effects of Cr (VI) on proliferation and oxidative stress-related indicators in Drosophila S2 cells and to explore the relationship between Cr (VI) and Drosophila S2 cells in order to provide theoretical basis for studying the toxic effects of Cr (VI) on Cr (VI) Methods Drosophila S2 cells were cultured in culture medium containing different concentrations of Cr (VI) (50, 100, 200, 400 and 800 μmol / L). CCK-8 assay was used to detect the effect of different concentrations of Cr Proliferation; the content of SOD and MDA in supernatant of culture medium was detected by spectrophotometry. Results As the concentration of Cr (VI) increased, the survival rate of S2 cells decreased and there was a significant dose - dependent relationship. The inhibitory rate of Cr (VI) on S2 cells reached as high as (88.51 ± 0.72)% at 800 μmol / L. Compared with the control group, the SOD activity had a significant difference (P <0.05) from 200μmol / L, and decreased gradually with the increase of Cr (VI) concentration. The content of MDA Compared with the control group, the differences were statistically significant (P <0.05). With the increasing concentration of Cr (VI), the content of MDA gradually increased. Conclusion Cr (VI) can significantly inhibit the proliferation of S2 cells in a dose-dependent manner. Cr (VI) can cause oxidative stress in S2 cells. Oxidative stress may be one of the important reasons for the inhibition of proliferation of S2 cells by Cr (VI).