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细胞膜特性是活细胞研究指标中的一项重要标记物,包括细胞膜杨氏模量、膜表面张力、膜上离子通道机械门控性等多种属性.这些属性在触摸、刺痛等机械力刺激信号介导的感知系统中起着重要的作用.然而,由于缺乏有效的研究工具和手段,现阶段对细胞膜特性的研究还并不广泛、深入.为此,本文将平面膜片钳技术与纳米操作机器人技术相集成,建立了一套能够对细胞施加超微机械力刺激并同步检测细胞电生理信号与物理特性变化的细胞多维信息检测系统.该系统主要包括平面膜片钳、纳米操作机器人与微流控系统3部分,能够实现细胞封接、细胞内液更换和信号采集与处理的自动化操作.利用该综合系统,本文深入研究了不同钳制电压对神经瘤Neuro-2a细胞膜杨氏模量的影响,实验结果表明随着钳制电压的增大,细胞膜杨氏模量逐渐减小.理论分析结果与实验一致.
Cell membrane properties are important markers in living cell research, including properties such as Young’s modulus of the cell membrane, membrane surface tension, mechanical gating of the ion channels on the membrane, etc. These properties are stimulated by mechanical stimuli such as touch and tingling Signal-mediated sensing system plays an important role.However, due to the lack of effective research tools and means, the research on the characteristics of cell membrane is not extensive and in-depth at this stage.Therefore, in this paper, planar patch- A robotic multi-dimensional information detection system was established to manipulate the cells and to detect the change of electrophysiological signals and physical characteristics of the cells by using the micromechanical force to stimulate the cells. The system mainly includes planar patch-clamp, nanomanipulator and Microfluidic system 3 part, can realize cell sealing, intracellular fluid exchange and signal acquisition and processing automation operation.Using the integrated system, this paper in-depth study of different clamping voltage on neurofilament Neuro-2a cell membrane Young’s modulus The experimental results show that the Young’s modulus of the cell membrane decreases with the increase of the clamping voltage.The theoretical analysis results are consistent with the experiment.