论文部分内容阅读
本文旨在探讨从人脐带静脉血是否可分离出基质干细胞,如果可以,确定它们的特性,方法是采用不支持造血干细胞生长的培养基培养脐血单个核细胞,对其生物学特性进行研究。1资料与方法1.112份脐血采至厦门市中山医院,每份30~60ml。1.2基质细胞的分离与培养经新生儿父母同意,于厦门市中
This article aims to investigate whether stromal stem cells can be isolated from human umbilical cord blood and, if so, to determine their characteristics by culturing cord blood mononuclear cells using a medium that does not support hematopoietic stem cell growth and studying its biological properties. 1 Materials and Methods 1.112 copies of cord blood collected to Zhongshan Hospital, Xiamen, each 30 ~ 60ml. 1.2 stromal cell isolation and culture consent of the newborn parents, in Xiamen City