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目的 :通过对肾移植病人的配组淋巴细胞毒抗体 (panelreactiveantibodyPRA)的检测 ,了解与分析肾移植病人配组淋巴细胞毒抗体的差异。方法 :以 36名健康成人外周血淋巴细胞作为群体淋巴细胞来源。采用补体依赖微量淋巴细胞毒方法 ,检测病人血清中的配组淋巴细胞毒抗体。结果 :55例病人进行血清淋巴细胞毒交叉配合实验检测 ,只有 1例阳性 1.82 %。而PRA检测 8例病人血清为阳性 (6女 2男 )占 14.5%。同时此群体中 35例病人曾经输血 ,其中PRA阳性有 6例(17.1% ) ,而未经输血病人 2 0例 ,其中有 2例PRA阳性 (10 % )。结论 :男女病人的PRA结果有显著性差异 (P <0 .0 5)。而输血与未输血病人FRA值无显著性差异 (P >0 .0 5)。与淋巴细胞毒交叉配合实验对比较 ,PRA的检测能更准确预测病人体内致敏状况和抗体水平。
OBJECTIVE: To detect and analyze the difference of lymphocyte cytotoxic antibody in renal allograft patients by detecting panelreactive antibody PRA in renal transplant patients. Methods: Thirty-six healthy adult peripheral blood lymphocytes were used as a source of population lymphocytes. Using complement-dependent microlymphocytotoxicity assay, the patient’s serum with the group of lymphocyte antibodies detected. Results: 55 cases of patients with serum lymphocyte cross-mating test, only 1 positive 1.82%. Serum samples from 8 patients with PRA were positive (6 males and 2 males) accounting for 14.5%. At the same time, 35 patients in this cohort had blood transfusion, of which PRA was positive in 6 cases (17.1%), while 20 cases were not transfused, of which 2 were PRA positive (10%). Conclusion: There is a significant difference in PRA between male and female patients (P <0.05). There was no significant difference in FRA between transfused and non-transfused patients (P> 0.05). Compared with the cytotoxicity test of lymphocytes, the PRA test can predict the sensitization status and antibody level of the patients more accurately.