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目的探讨促炎细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)、IL-6在老年感染性多器官损伤中的意义。方法将大鼠随机分为青年对照组、青年模型组和老龄对照组、老龄模型组;采用气管插管法注入肺炎克雷们杆菌,由肺炎导致多器官损伤;应用光学显微镜、免疫组织化学技术,观察肺、心、小肠及肾脏组织病理改变与促炎细胞因子TNF-α、IL-1、IL-6变化情况。结果与同龄对照组比较,老龄模型组和青年模型组肺、心、肾、小肠组织TNF-α、IL-1、IL-6表达水平明显增高及脏器组织病理学改变明显(P<0.01或P<0.05);而老龄模型组肺IL-1、心TNF-α、IL-6和小肠IL-1、IL-6表达水平又较青年模型组显著增高(P<0.01或P<0.05),脏器损伤亦较重(P<0.05)。结论促炎细胞因子TNF-α、IL-1、IL-6参与了老年感染性多器官损伤,并在其中发挥了重要作用。
Objective To investigate the significance of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6) Methods The rats were randomly divided into young control group, young model group, aging control group and aging model group. Injecting Klebsiella pneumoniae by tracheal intubation and multiple organ injury by pneumonia. Using light microscope, immunohistochemistry The pathological changes of lung, heart, small intestine and kidney and the changes of proinflammatory cytokines TNF-α, IL-1 and IL-6 were observed. Results Compared with the control group at the same age, the expression of TNF-α, IL-1 and IL-6 in lung, heart, kidney and small intestine of aged model group and young model group were significantly increased and histopathological changes were significantly (P <0.01 or P <0.05). The levels of IL-1, TNF-α, IL-6 and IL-1 and IL-6 in the intestinal mucosa in aged model group were significantly higher than those in the young model group (P <0.01 or P < Organ damage is also heavier (P <0.05). Conclusions The proinflammatory cytokines TNF-α, IL-1 and IL-6 are involved in the pathogenesis of senile infectious multiple organ injury and play an important role in them.