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目的:利用响应面法对红花中红花黄色素的超声提取工艺进行优化。方法:通过单因素试验选取试验因素与水平;根据中心组合试验设计原理,以羟基红花黄色素A为指标成分,以其提取率为响应值,采用三因素三水平的响应面法建立数学模型并获得最优提取条件。结果:红花黄色素的最佳提取工艺为:超声温度55℃,液料比16,超声时间39 min,提取3次。结论:在此条件下,红花黄色素的提取率为1.798%,与理论预测值1.849%的相对误差为2.758%。优化后的方法可为高效提取红花中的红花黄色素提供参考。
Objective: To optimize the ultrasonic extraction process of safflower yellow pigment by using response surface methodology. Methods: The factors and levels of test were selected by single factor test. According to the design principle of central composite test, hydroxysafflor yellow A was taken as the index component and the extraction rate was used as the response value. The mathematical model was established by the response surface method of three factors and three levels And obtain the optimal extraction conditions. Results: The optimum extraction process of safflower yellow pigment was as follows: the ultrasonic temperature was 55 ℃, the ratio of liquid to material was 16, the time of ultrasonic was 39 min, and the extraction time was 3 times. Conclusion: Under this condition, the extraction rate of safflower yellow pigment is 1.798%, and the relative error with theoretical prediction value of 1.849% is 2.758%. The optimized method can provide reference for the efficient extraction of safflower yellow pigment in safflower.