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根据病原菌的生物学特性、生理生化特性包括革兰氏染色反应、氧化酶反应、过氧化氢酶反应、氧需求等和特异性PCR扩增结果,以及病原菌危害造成的田间症状,发现燕麦嗜酸菌属西瓜亚种(Acidovoraxavenaesubsp.Citrulli)可在新寄主-籽瓜上造成严重危害。该病菌在田间主要侵染籽瓜的果实和子叶,症状在果实上尤为明显,形成黑色的星状开裂。为了控制该病的发生,对种子携带病原菌的快速检测方法进行了研究。结果表明,特异性PCR作为检测种传病原菌具有快速、准确和灵敏的特点。当种子浸出液为PCR反应的模板时,可检测出的种子带菌率极限最低为4%;当以种子浸出液提取的DNA为模板时,种子带菌率检测极限为2%甚至更低。
According to the biological characteristics of pathogenic bacteria, physiological and biochemical characteristics include Gram stain, oxidase, catalase, oxygen demand and specific PCR amplification results, as well as pathogenic bacteria caused by the field symptoms, found oat acid Acidovoraxavenaesubsp. Citrulli can cause serious damage on the new host, the seed-cucumber. The bacteria mainly in the field of seed and melon seeds and cotyledons, the symptoms are particularly evident in the fruit, the formation of black star-shaped cracking. In order to control the occurrence of the disease, rapid detection of pathogen carrying seeds was studied. The results showed that specific PCR as a detection of pathogen pathogens with fast, accurate and sensitive characteristics. When the seed leachate was used as a template for PCR reaction, the lowest limit of detectable seed germination rate was 4%. When the DNA extracted from seed leachate was used as a template, the detection limit of seed germination rate was 2% or less.