用高效农相色谱法测定补肾益脑胶囊中科骨脂素和异补骨脂素含量，以控制该制剂的质量。方法：用十八烷基健合硅胶柱分离科骨脂素和异补骨脂素，以甲醇-水（45:55）为流动相，检测波长245nm。结果：补膏脂素和异补膏脂素峰与其它组分峰的分高度为1．2，理论塔板数以补膏脂素和异科骨脂素峰计算均为4700；线性范围：补膏脂素0．022～0.176μg，异补骨脂素0．0204～0．163μg。补膏脂素平均回收率101．0％，RSD＝2.44％（n=6），异补膏脂素平均回收率101.4％，RSD=2．73％（n=6）。结论：本法简便、快速，适用于该产品质量分析检验。 The contents of sulfatine and isopsoralen in Bushenyinnao Capsules were determined by high performance agrochemical chromatography to control the quality of the formulation. Methods: Ketosorcin and isopsoralen were separated by octadecyl-silica gel column. The mobile phase was methanol-water (45:55). The detection wavelength was 245nm. RESULTS: The peak heights of the peaks of the ointment and ibuprost and other components were 1.2, and the theoretical plate number was 4700 for both ointment and isoflavone peaks. Linear range: Filling cream 0.022 ~ 0.176μg isopsoralen 0.0204 ~ 0.163μg. The average recovery rate of pectin was 101.0%, RSD was 2.44% (n=6), the average recovery rate of isosorbide was 101.4%, and RSD was 2.73% (n=6). Conclusion: This method is simple, rapid and applicable to the quality analysis of the product.