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目的:研究致肾盂肾炎大肠埃希菌(UPEC)132对细胞的粘附和侵袭能力。方法:对比致病菌株UPEC132及无菌毛代表菌株E.coli K-12 p678-54对Vero、Ketr-3及EJ细胞的粘附率、粘附指数和侵袭指数。结果:E.coli K-12p678-54对此3种细胞无粘附无侵袭,而UPEC132对其有明显作用,可致细胞形态明显改变直至死亡。UPEC132对Vero、Ketr-3及EJ细胞的粘附率分别为(61.44±3.21)%、(55.22±4.09)%和(58.67±5.12)%,差别无统计学意义;对3种细胞的粘附指数分别为1.44±0.06、1.74±0.09和2.27±0.18,有显著性差异(P<0.05)。UPEC132对EJ和Ketr-3细胞的侵袭指数分别为(3.25±0.20)×10-3和(3.00±0.34)×10-3,两者之间无统计学差异,但均高于对Vero细胞的侵袭指数[(2.61±0.32)×10-3,P<0.05]。结论:UPEC132对Vero、Ketr-3、EJ细胞均有粘附和侵袭能力,其中对EJ细胞的粘附能力最强,侵袭力也较Vero细胞强,可利用该细胞深入研究UPEC132的毒力及致病机制。
PURPOSE: To investigate the ability of UPEC 132 to adhere and invade cells. Methods: The adhesion rate, adhesion index and invasion index of Vero, Ketr-3 and EJ cells were compared between the strains UPEC132 and E.coli K-12 p678-54. Results: E.coli K-12p678-54 had no invasion and no invasion to the three kinds of cells, but UPEC132 had a significant effect on it, which caused the cell morphology to change significantly until death. The adhesion rates of UPEC132 to Vero, Ketr-3 and EJ cells were (61.44 ± 3.21)%, (55.22 ± 4.09)% and (58.67 ± 5.12)%, respectively, with no significant difference. The indices were 1.44 ± 0.06, 1.74 ± 0.09 and 2.27 ± 0.18 respectively, with significant difference (P <0.05). The invasion index of UPEC132 to EJ and Ketr-3 cells were (3.25 ± 0.20) × 10-3 and (3.00 ± 0.34) × 10-3, respectively, but there was no significant difference between them Invasion index [(2.61 ± 0.32) × 10-3, P <0.05]. CONCLUSION: UPEC132 has the ability of adhesion and invasion to Vero, Ketr-3 and EJ cells. The adhesion ability of UPEC132 to EJ cells is the strongest and the invasion ability is stronger than that of Vero cells. UPEC132 can be used to study the virulence of UPEC132 Disease mechanism.