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以IR71331为材料,通过水培试验,采用双向电泳分离不同磷浓度下(低磷浓度为0.5mg·L-1,对照为10mg·L-1)水稻生长3d和6d根系差异蛋白.结果表明:与对照相比,低磷胁迫下共有29个蛋白,其中3d时间点有17个蛋白上调、11个下调、1个新增,6d时间点有8个上调、19个下调、1个抑制表达、1个无明显变化.经鉴定,其中的10个差异表达蛋白可归为信号转导相关蛋白、基因表达相关蛋白、代谢相关蛋白离子转运相关蛋白4个功能类群.信号转导相关蛋白分别为富含甘氨酸RNA结合蛋白和类似参与磷酸盐饥饿反应调控子;基因表达相关蛋白分别为推定的mRNA前体剪接因子SF2和推定的AAA蛋白酶家族FtsH;代谢相关蛋白分别为腺苷酸琥珀酸裂解酶、丝氨酸蛋白酶抑制剂(serpin)、S-腺苷蛋氨酸合成酶(SAM)和类似MYB类转录因子;离子转运相关蛋白分别为阳离子转运ATP酶和肌浆网膜蛋白.这些蛋白分别参与了信号识别、信号调控、mRNA的剪接、信号传递、蛋白质降解、细胞体内离子转运和平衡等生理过程.其中serpin、SAM和MYB类转录因子是水稻响应低磷胁迫的关键蛋白.水稻根系对低磷胁迫存在着一个复杂的抗逆信号应答和代谢调控网络,其作用机理可以通过差异表达的蛋白质得以体现.
IR71331 was used as material to separate the three-dimensional and six-day differential proteins at different phosphorus concentrations (0.5 mg · L-1 for low phosphorus and 10 mg · L-1 for rice) by two-dimensional electrophoresis. Compared with the control, there were 29 proteins under low-phosphorus stress, of which 17 proteins were up-regulated, 11 were down-regulated and 1 was new at 3d, 8 were up-regulated at 6 d, 19 were down-regulated and 1 was down- One of the 10 differentially expressed proteins was identified as four functional groups of signal transduction related proteins, gene expression related proteins and metabolism related protein ion transport related proteins, and the signal transduction related proteins were rich Glycine-containing RNA binding protein and similar regulator involved in phosphate starvation response; gene expression related proteins were putative pre-mRNA splicing factor SF2 and presumed AAA protease family FtsH; metabolism-related proteins were adenosylsuccinate lyase, Serpin, SAM and similar MYB transcription factors, and ion transport related proteins are cation transport ATPase and sarcoplasmic reticulum proteins, respectively Signal transduction, signal regulation, mRNA splicing, signal transduction, protein degradation, intracellular ion transport and balance and other physiological processes were involved in this study, among which serpin, SAM and MYB transcription factors were the key proteins in response to low P stress. There is a complex network of stress response and metabolic regulation in phosphorus stress, and its mechanism of action can be expressed by differentially expressed proteins.