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目的探讨压力蒸汽灭菌器生物学监测自含式生物指示剂颜色异常原因,合理判定监测结果。方法通过现场调查,查找原因,同时进行实验室检测,以明确结果。结果生物学监测培养结果颜色异常是因灭菌器冷空气排除困难,预热高温时间延长,培养基破坏所致;对颜色异常的培养管中的菌片进行实验室培养无菌生长。结论压力蒸汽灭菌器冷空气排除困难要关注疏水阀。异常的标本实验室检测无菌生长,仍应查找异常原因,不能简单判定灭菌合格而不做处理。
Objective To investigate the causes of color abnormalities in self-contained biological indicator for biological monitoring of pressure steam sterilizer and reasonably determine the monitoring results. Methods By field investigation, find out the reasons, at the same time, laboratory tests to determine the results. Results The results of biological monitoring showed that the color abnormalities were caused by the cold air of the sterilizer being difficult to remove, the preheating and high temperature prolonged, and the destruction of the medium. The bacteria in the abnormal color culture tubes were aseptically grown in laboratory. Conclusion Pressure steam sterilizer cold air removal difficulties should pay attention to traps. Abnormal specimen laboratory testing of aseptic growth, should still find the cause of the anomaly, can not simply determine the sterilization qualified without treatment.