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[Objective] To optimize the purification conditions of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE by macroporous resins.[Method] 5 kinds of macroporous resins including ADS-7,HPD-300,D-101,HPD-910 and AB-8 were adopted to adsorb flavonoids from RADIX RHIZOMA GLYCYRRHIZAE.Besides,effects of factors affecting the adsorption properties such as sample concentration,ethanol concentration and ethanol volume were also investigated.[Result] ADS-7 was provided with higher absorption rate and the elution rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE,the optimum effect of which were obtained with sample concentration at 1.2 mg/ml using 5 BV of 70% ethanol as eluent.After purification,the content and refined rate of flavonoids in powder were 47.1% and 211.2%,respectively.[Conclusion] ADS-7 resin had strong adsorption and purification effects,which would provide reference basis for purifying flavonoids from RADIX RHIZOMA GLYCYRRHIZAE.
[Objective] To optimize the purification conditions of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE by macroporous resins. [Method] 5 kinds of macroporous resins including ADS-7, HPD-300, D-101, HPD-910 and AB- flavonoids from RADIX RHIZOMA GLYCYRRHIZAE.Besides, effects of factors affecting the adsorption properties such as sample concentration, ethanol concentration and ethanol volume were also investigated. [Result] ADS-7 was provided with higher absorption rate and the elution rate of flavonoids from RADIX RHIZOMA GLYCYRRHIZAE, the optimum effect of which were obtained with sample concentration at 1.2 mg / ml using 5 BV of 70% ethanol as eluent. After purification, the content and refined rate of flavonoids in powder were 47.1% and 211.2%, respectively. ] ADS-7 resin had strong adsorption and purification effects, which would provide reference basis for purifying flavonoids from RADIX RHIZOMA GLYCYRRHIZAE.