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目的:建立测定妇炎康复胶囊中橙皮苷和黄芩苷含量的HPLC测定方法。方法:采用Shim-pack VP-ODS柱,乙腈-0.5%磷酸(25:75)为流动相,检测波长为283nm,流速1.0ml·min~(-1)。结果:橙皮苷在0.13～0.38μg范围内与峰面积呈良好线性关系,r=1.000 0,平均加样回收率为99.53%,RSD为1.1%(n=6);黄芩苷在0.59～1.76μg范围内与峰面积呈良好线性关系,r=1.000 0,平均加样回收率为99.55%,RSD为1.3%(n=6)。结论:该方法简便、准确,专属性强,重现性好,可用于妇炎康复胶囊的质量控制。
Objective: To establish an HPLC method for determination of hesperidin and baicalin in fuyan rehabilitation capsules. Methods: A Shim-pack VP-ODS column was used with acetonitrile-0.5% phosphoric acid (25:75) as the mobile phase. The detection wavelength was 283 nm and the flow rate was 1.0 ml·min -1 . Results: Hesperidin showed a good linear relationship with the peak area in the range of 0.13-0.38 μg, r=1.000 0, the average recovery was 99.53%, RSD was 1.1% (n=6), and baicalin was 0.59-1.76. Within the range of μg, there was a good linear relationship with the area of the peak, r=1.000 0, the average recovery was 99.55%, and the RSD was 1.3% (n=6). Conclusion: This method is simple, accurate, specific and reproducible. It can be used for the quality control of Fuyan rehabilitation capsule.