一般情况下菇类菌种组织培养复壮，将未喷水长至７成左右，菇形较好的菇采下，用７５％酒精或其它消毒液进行表面消毒灭菌，再用无菌水冲洗，取菌盖与柄交界处一小块组织接在ＰＤＡ培养基上培养。由于表面未杀死的部分杂菌渗入到组织内，所取组织易带杂菌，成功率低。 笔者将菇 Under normal circumstances mushroom culture tissue rejuvenation, no water will grow to about 7 percent, Mushroom-shaped mushroom mining, with 75% alcohol or other disinfectant surface disinfection and sterilization with sterile water , Take a small piece of tissue at the junction of the cap and the handle connected to the PDA medium culture. As part of the surface of the bacteria did not kill infiltration into the organization, the organization taken easily bacteria, the success rate is low. I will mushroom