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为进一步了解缺氮对油菜养分吸收的影响,以甘蓝型油菜中双11号为材料,采用营养液培养方法,设置正常供氮和缺氮两个处理,培养14d后收获,分析缺氮对油菜植株表型、生物量、植株养分含量及养分吸收相关基因表达的影响。结果表明,缺氮严重抑制油菜植株生长,地上部生物量显著减少,叶片瘦小、黄化,茎秆呈紫红色。缺氮促进油菜根系生长,植株根冠比增加。缺氮处理对油菜叶、茎、根中大量元素含量的影响有所不同,缺氮油菜叶、茎、根的氮浓度及氮含量都显著降低;油菜叶、茎的磷浓度在缺氮胁迫时显著增加,根的磷浓度显著降低,而茎和根的磷含量在缺氮胁迫时都显著降低,但叶片磷含量不受影响;油菜不同部位钾浓度和钾含量在缺氮胁迫时都显著降低,并且缺氮处理14d显著抑制油菜大部分钾通道蛋白基因以及3个根部特异钾转运蛋白基因的表达,说明缺氮对油菜钾吸收运转相关基因表达的影响,可能导致油菜植株钾含量的降低。
In order to further understand the effect of nitrogen deficiency on nutrient uptake in rapeseed (Brassica napus), Zhongshu 11 was used as material, and nutrient solution culture method was used to set up normal nitrogen supply and nitrogen deficiency treatment. After 14 days cultivation, Plant Phenotype, Biomass, Plant Nutrient Content and Nutrient Absorption Related Gene Expression. The results showed that nitrogen deficiency severely inhibited the plant growth of rapeseed and significantly reduced shoot biomass. The leaves were thin and yellow with purple stalk. Nitrogen deficiency promoted root growth of rapeseed and root / shoot ratio increased. The effects of nitrogen deficiency on the contents of many elements in rape leaves, stems and roots were different. The nitrogen and nitrogen contents of leaves, stems and roots of rape were significantly decreased. The phosphorus content in roots and roots decreased significantly under nitrogen stress, but the phosphorus content in leaves was not affected. The potassium and potassium contents in different parts of rape were significantly decreased under nitrogen stress , And nitrogen deficiency significantly inhibited the expression of most potassium channel genes and three root specific potassium transporter genes in rapeseed, indicating that the effect of nitrogen deficiency on rape potassium uptake may reduce the content of potassium in rapeseed.