为探讨不同浓度硒对神经细胞增殖和分化的影响 ,将细胞培养物经不同浓度 (0 0 1～ 10 μmol/L)亚硒酸钠(Na2 SeO3)处理 ,用MTT法检测细胞增殖率 ,并观察细胞形态变化和细胞分化程度。结果 :低浓度硒 (0 0 1～ 0 1μmol/L)能促进神经细胞增殖和分化 ;较高浓度硒 (0 0 5～ 5 μmol/L)则抑制神经细胞增殖和分化 ,并均呈剂量 -效应关系 ,高浓度硒(10 μmol/L)引起细胞毒性。提示低浓度硒有助于分化期大鼠胚胎神经细胞增殖和分化。 In order to investigate the effect of different concentrations of selenium on the proliferation and differentiation of neural cells, the cell cultures were treated with different concentrations of sodium selenite (0.01-0.10 μmol / L), the cell proliferation rate was measured by MTT assay Observation of cell morphology and cell differentiation. RESULTS: Selenium (0 0 1 ~ 0 1μmol / L) could promote the proliferation and differentiation of neural cells. Higher concentrations of selenium (0 05 ~ 5 μmol / L) inhibited the proliferation and differentiation of nerve cells, Effect, high concentrations of selenium (10 μmol / L) cause cytotoxicity. Suggest that low concentrations of selenium contribute to the differentiation of rat embryonic neural cells proliferation and differentiation.