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目的 探讨碱性成纤维细胞生长因子 (b FGF)治疗新生大鼠缺氧缺血性脑损伤(HIBD)的作用机制。方法 将 72只 7日龄 Wistar大鼠随机分为三组 ,即假手术对照组 (8只 ) ,HIBD组 (32只 ) ,b FGF治疗组 (32只 ) ,后两组常规制成 HIBD模型 ,治疗组每日给b FGF腹腔注射 ,监测不同时间脑组织中 SOD、MDA、及 NO水平。结果 与假手术组相比 ,HIBD组脑组织中 SOD明显降低 ,而 MDA、NO明显升高 ,b FGF治疗组与生理盐水对照组比较 ,有不同程度的 SOD升高及 MDA、NO降低 ,且差异均显著。结论 氧自由基与 NO参与了HIBD的发病过程 ,b FGF可促进缺氧缺血后脑组织中 SOD水平的升高 ,降低自由基水平 ,在HIBD的防治方面可能具有一定的作用
Objective To investigate the mechanism of basic fibroblast growth factor (b FGF) in the treatment of hypoxic-ischemic brain damage (HIBD) in neonatal rats. Methods 72 7-day-old Wistar rats were randomly divided into three groups: sham operation control group (n = 8), HIBD group (n = 32) and b FGF treatment group . The treatment group was intraperitoneally injected with bFGF daily to detect the levels of SOD, MDA and NO in brain tissue at different time points. Results Compared with sham operation group, SOD in HIBD group decreased significantly and MDA and NO increased significantly. Compared with saline control group, bFGF group increased SOD and MDA and NO decreased The difference was significant. Conclusions Oxygen free radicals and NO participate in the pathogenesis of HIBD. B FGF can promote the increase of SOD level and decrease the level of free radicals in the brain tissue after hypoxic-ischemia, which may play a role in the prevention and treatment of HIBD