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目的探讨COX2选择性抑制剂NS398对食管癌细胞株EC9706放射敏感性的影响及作用机制。方法选用高表达COX2的人食管癌细胞株EC9706为靶细胞,加入不同浓度(10、20、50、100μmol/L)的NS398,分别作用24、48h,用直线加速器分别给予0、2、4、6、8、10Gy剂量照射,采用成克隆实验分析增敏效应,用DNA片段分析法及流式细胞术检测细胞凋亡情况。结果用10、20、50、100μmol/L的NS398作用24、48h均能检测到放射增敏作用,其放射增敏比(Dq比)分别为1.11、1.24、1.40、1.54和1.11、1.27、1.58、1.67,呈剂量和时间依赖性;同时使细胞凋亡率增加。结论NS398能增强人食管癌细胞株EC9706对放射的敏感性,其增敏机制与抑制亚致死性损伤修复和诱导细胞凋亡增加有关。
Objective To investigate the effect and mechanism of COX2 selective inhibitor NS398 on radiosensitivity of esophageal carcinoma cell line EC9706. Methods Human esophageal cancer cell line EC9706 with high expression of COX2 was selected as target cells. NS398 cells were treated with different concentrations (10, 20, 50 and 100 μmol / L) for 24 and 48 hours, respectively. 6,8,10 Gy dose irradiation, the use of cloning experiments to analyze the sensitization effect, DNA fragmentation analysis and flow cytometry were used to detect apoptosis. Results Radiosensitization was detected at 24 h and 48 h after treatment with 10, 20, 50 and 100 μmol / L NS398, respectively. The radiosensitivities (Dq ratios) were 1.11, 1.24, 1.40, 1.54 and 1.11, , 1.67, in a dose-and time-dependent manner; while increasing the rate of apoptosis. Conclusion NS398 can enhance the sensitivity of human esophageal carcinoma cell line EC9706 to radiation. The mechanism of sensitization to NS398 is related to the inhibition of sublethal injury repair and the induction of apoptosis.