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目的:比较不同浓度NaCl处理下药用菊花红心菊和黄菊,以及二者通过正交与反交获得的F1代植株的耐盐性,分析其耐盐性的遗传机制以及耐盐性与黄酮、绿原酸等主要活性成分的相关性。方法:将材料置于加入5种不同浓度(0,40,80,120,160 mmol.L-1)NaCl的Hoagland培养液中进行胁迫处理,每日增加NaCl浓度并保证浓度梯度,一段时间后测定受伤害叶面积比率、脯氨酸、甜菜碱、丙二醛、黄酮、绿原酸的含量并分析。结果:NaCl浓度在120 mmol.L-1以下时,红心菊耐盐性高于黄菊,红心菊×黄菊耐盐性高于亲本,黄菊×红心菊表现介于亲本之间;NaCl浓度在120～160 mmol.L-1时,黄菊耐盐性高于红心菊,黄菊×红心菊耐盐性高于亲本而红心菊×黄菊表现介于亲本之间。结论:F1代遗传性受母本影响较多,黄酮、绿原酸含量与植株耐盐性表现出一定相关性。
OBJECTIVE: To compare the salt tolerance of the F1 plants from two medicinal plants, Chrysanthemum indicum and Chrysanthemum indicum, treated with different concentrations of NaCl, and to analyze the genetic mechanism of salt tolerance and the effects of salt tolerance on flavonoids, Chlorogenic acid and other major active ingredients of relevance. Methods: The materials were placed in Hoagland medium with 5 different concentrations (0, 40, 80, 120, 160 mmol.L-1) of NaCl for stress treatment. NaCl concentrations were increased daily to ensure the concentration gradient. Injured leaves Area ratio, proline, betaine, malondialdehyde, flavonoids, chlorogenic acid content and analysis. Results: The salt tolerance of Radix Hedysari was higher than that of Huangju when the concentration of NaCl was below 120mmol.L-1, the salt tolerance of Radix Aconiti Kusnezoffii × Chrysanthemum was higher than that of the parent, and the content of NaCl was 120 ~ 160 mmol.L-1, the salt tolerance of chamomile was higher than that of heart, the salt tolerance of chamomile × chamaejasme was higher than that of parents, and the content of chamaejurum × chamomile was between that of parents. CONCLUSION: The inheritance of F1 generation is more affected by the female parent. The contents of flavonoids and chlorogenic acid are related to the salt tolerance of the plants.