目的设计特异性聚合酶链式反应(PCR)引物,建立川麦冬PCR鉴定方法。方法回收随机扩增多态性DNA标记(RAPD)扩增筛选到的川麦冬分子标记CM503基因,将其连接进入T-载体克隆并测序。根据测序结果设计一对特异性引物CM1/CM2,以麦冬基因组DNA为模板进行特异性PCR扩增。结果 PCR鉴定结果为在退火温度68℃时,川麦冬在297 bp处出现特异性扩增条带,其他麦冬品种则未出现条带。结论实验室建立的川麦冬PCR鉴定方法具有操作简单、准确、灵敏、重复性好等优点,应用前景理想。 Objective To design specific polymerase chain reaction (PCR) primers and establish a method for PCR identification of Chuan. Methods The CM503 gene was amplified by random amplified polymorphic DNA (RAPD) amplification and cloned into T-vector and sequenced. According to the sequencing results, a pair of specific primers CM1 / CM2 was designed and the genomic DNA of Ophiopogon japonicus was used as a template for specific PCR amplification. The results of PCR identification results showed that when the annealing temperature was 68 ℃, Chlamys farreri displayed a specific amplified band at 297 bp, while the other bands did not show bands. Conclusion The method of PCR identification established by the laboratory is simple, accurate, sensitive and reproducible.