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目的 用分子生物学技术鉴定西沙群岛恙虫病东方体的基因序列 ,探讨南海岛屿恙虫病疫源地的形成。方法 以巢式聚合酶链反应 (NPCR)检测西沙群岛恙螨所携恙虫病东方体的 5 6kDa蛋白基因片段 ,继而将NPCR产物克隆进pGEM-T载体并且测序 ,测序结果在国际互联网作多序列比较和进化树分析。结果 从西沙群岛收集的恙螨扩增出 5 0 7bp目的片段 ,序列分析证实与Karp株同源性 85 % ,与Gilliam株同源性 6 8% ,与Yonchon株同源性 6 7% ,与Kato株同源性 6 5 %。结论 西沙群岛的恙螨携带恙虫病东方体以Karp型为主。
Objective To identify the gene sequence of Orientia tsutsugamushi in the Xisha Islands by molecular biology techniques and discuss the formation of the tsutsugamushi disease in the South China Sea. Methods The 5 6 kDa protein gene fragment of Orientia tsutsugamushi in the Xisha Islands was detected by nested polymerase chain reaction (NPCR). Subsequently, the NPCR product was cloned into the pGEM-T vector and sequenced. The sequencing results were sequenced on the Internet for multiple sequences Comparison and evolutionary tree analysis. Results A fragment of 500bp was amplified from the chigger mites collected from the Xisha Islands. Sequence analysis confirmed 85% homology with Karp strain, 68% homology with Gilliam strain, 67% homology with Yonchon strain, and Kato strain homology 6 5%. Conclusion The Karp-type oriental body of the chigger mite carrying tsutsugamushi in Xisha Islands is predominant.