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用不同浓度胶原酶分离小鼠腔前卵泡，以４ ｍｇ／ ｍｌ 胶原酶处理组所获采集量大且对腔前卵泡早期培养无影响。培养液中分别加入２０ ％ 牛卵泡液、ＦＳＨ＋ ＬＨ（ 各１０ＩＵ／ ｍｌ） 和胰岛素（０ ．０１ｕｇ／ ｍｌ） ，结果加入２０ ％ 牛卵泡液组的腔前卵泡在培养１０ｄ 后卵泡直径明显大于对照组，而ＧＶＢＤ 发生率明显低于对照组；加入ＦＳＨ ＋ ＬＨ（ 各１０ＩＵ／ ｍｌ） 或胰岛素（０ ．０１ｕｇ／ ｍｌ） 组的腔前卵泡ＧＶＢＤ 发生率明显高于对照组。提示：ＦＳＨ 、ＬＨ、胰岛素能促进腔前卵泡体外成熟，卵泡液能促进腔前卵泡体外生长而抑制其体外成熟
Pre-parenchymal follicles were isolated from mice with different concentrations of collagenase and collected in 4 mg / ml collagenase group without any effect on early culture of preantral follicles. The follicles of 20% bovine follicular fluid, FSH + LH (each 10IU / ml) and insulin (0. 01ug / ml) were added into the culture medium. The diameter of follicle in 20% Group, while the incidence of GVBD was significantly lower than that of the control group. The incidence of GVBD in the preantral follicles of FSH + LH group (10 IU / ml) or insulin (0. 01 ug / ml) group was significantly higher than that of the control group. Tip: FSH, LH, insulin can promote the premature rupture of follicles in vitro, follicular fluid can promote preantral follicles in vitro growth and inhibit its in vitro maturation