论文部分内容阅读
目的检测乙醇胺的诱变性,为其安全性评价提供资料。方法采用3种致突变试验:鼠伤寒沙门菌回复突变试验(Ames试验),小鼠骨髓嗜多染红细胞微核试验和小鼠淋巴瘤细胞TK基因突变试验。Ames试验设剂量组为0.08、0.4、2.0、10.0和50.0μl/皿,另设空白、溶剂、阳性对照组;微核试验设剂量组为343.75、687.5和1 375.0 mg/kg,另设阴性、阳性对照;TK基因突变试验设剂量组为4、6、8和10 mmol/L,另设阴性、阳性对照组。结果 Ames试验中,加或不加S9的情况下,乙醇胺对TA97和TA100菌株有致突变作用。微核试验中,雌雄鼠微核率均呈现剂量-反应关系;雄鼠低、中两剂量组以及雌鼠中剂量组的微核率与阴性对照组比较,差异具有统计学意义。TK基因突变试验各剂量组突变频率与阴性对照比较,差异均无统计学意义。结论在本次实验条件下,测试剂量范围内,Ames试验、微核试验结果提示阳性,TK基因突变试验显示阴性。乙醇胺遗传毒性尚需结合其他检测系统进行探究和验证。
Objective To detect the mutagenicity of ethanolamine and provide information for its safety evaluation. Methods Three kinds of mutagenicity test were used: Salmonella typhimurium Ames test, mouse bone marrow polychromatic erythrocyte micronucleus test and mouse lymphoma TK gene mutation test. Ames test dose groups were 0.08,0.4,2.0,10.0 and 50.0μl / dish, the other a blank, solvent, positive control group; micronucleus test dose groups were 343.75,687.5 and 1375.0 mg / kg, another set of negative, Positive control; TK gene mutation test dose groups were 4,6,8 and 10 mmol / L, another set of negative, positive control group. Results In Ames test, ethanolamine induced mutagenesis on TA97 and TA100 strains with or without S9. In the micronucleus test, the micronucleus rate of the male and female mice showed a dose-response relationship. The micronucleus rate of the low-dose and middle-dose male rats and the middle-dose female rats were statistically different from the negative control group. There was no significant difference in the frequency of mutation in each dose of TK gene mutation test compared with the negative control. Conclusions Under this experimental condition, the results of Ames test and micronucleus test are positive and TK gene mutation test is negative within the range of test dose. Ethanolamine genotoxicity needs to be explored and validated in conjunction with other detection systems.