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目的观察地塞米松对实验性哮喘大鼠气道细胞ＤＮＡ合成和气道重塑的干预效果。 方法 应用ＳＤ大鼠建立哮喘动物模型，采用免疫组化技术和其它形态学研究的方法，研究雾化吸入地塞米松对气道细胞ＤＮＡ合成和气道重塑反应的影响。 结果 ① 地塞米松治疗组气道上皮下胶原沉积以及粘液的分泌比模型组明显减少。② 模型组气道平滑肌细胞Ｂｒｄｕ阳性计数（１０．２５±２．０９）明显高于正常对照组（７．１５±２．０５）和地塞米松治疗组（６．８５±２．２０）（Ｐ＜０．０１）；模型组气道上皮细胞Ｂｒｄｕ阳性计数（２１．８３±７．０１）亦明显高于正常对照组（１６．２２±４．３６）和地塞米松治疗组（１６．９２±３．４８）（Ｐ ＜ ０．０５）。 结论 应用地塞米松干预可减轻实验性哮喘大鼠气道炎症，抑制气道细胞ＤＮＡ合成，延缓气道重塑反应的发生。
Objective To observe the effect of dexamethasone on DNA synthesis and airway remodeling of airway cells in asthmatic rats. Methods SD rats were used to establish animal models of asthma. Immunohistochemistry and other morphological methods were used to study the effects of aerosolized dexamethasone on DNA synthesis and airway remodeling in airway cells. Results ① Dexamethasone treatment group, airway epithelial collagen deposition and mucus secretion was significantly reduced than the model group. ② The Brdu positive count (10.25 ± 2.09) in airway smooth muscle cells in model group was significantly higher than that in normal control group (7.15 ± 2.05) and dexamethasone group (6.85 ± 2.20) ( P <0.01). The Brdu positive count (21.83 ± 7.01) in model group was also significantly higher than that in normal control group (16.22 ± 4.36) and dexamethasone group .92 ± 3.48) (P <0.05). Conclusion Dexamethasone can reduce airway inflammation, inhibit DNA synthesis in airway cells and delay airway remodeling in experimental asthmatic rats.