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对淋巴毒素(LT)或肿瘤坏死因子-β(TNF-β)是否参与胆脂瘤病变过程进行研究,试图证明人中耳胆脂瘤组织中LT的部位和LT对基底角化细胞特性的影响,以便阐明LT在胆脂瘤发展中可能起的作用。标本从病人术中取得中耳胆脂瘤(12)和正常外耳道皮肤(5),制成6μm厚的石蜡切片,以兔抗人体LT IgG用间接免疫过氧化物酶法染色。在胆脂瘤组织的细胞间区和上皮细胞表面发现染成带棕色的LT,在胆脂瘤上皮下组织,LT存在于淋巴细胞和单核细胞中。正常外耳道皮肤的鳞状上皮染色较胆脂瘤上皮淡得多。在胆脂瘤上皮和肉芽组织中发现LT,可以设想胆脂瘤上皮中的LT来自肉芽组织中淋巴细胞。另一方面,在中耳炎症反应中上皮细胞有产生自身LT的能力,
A study of the involvement of lymphotoxin (LT) or tumor necrosis factor-β (TNF-β) in the pathogenesis of cholesteatoma attempts to demonstrate the effect of LT site and LT on the keratinocyte characteristics in human middle ear cholesteatoma In order to clarify the possible role of LT in the development of cholesteatoma. Specimens were obtained from patients with middle ear cholesteatoma (12) and normal external auditory canal skin (5), made into 6μm thick paraffin sections and stained with rabbit anti-human LT IgG by indirect immunoperoxidase. In the cholesteatoma tissue cells and epithelial cells found on the surface stained with brown LT, in the subcutaneous tissue of cholesteatoma, LT exists in lymphocytes and monocytes. Squamous epithelium of normal external auditory canal skin is much fairer than cholesteatoma epithelium. LT is found in cholesteatoma epithelium and granulation tissue, and it is conceivable that the LT in the cholesteatoma epithelium is derived from lymphocytes in granulation tissue. On the other hand, epithelial cells have the ability to produce their own LT in the inflammatory reaction of the middle ear,