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为了解福寿螺耐寒性是否存在性别差异,对华南地区的雌雄福寿螺进行了过冷却研究及低温胁迫下存活率调查,并对入冬前后其体内耐寒性生理生化物质进行测定.结果表明:雌雄螺的过冷却点均值分别为-6.83℃和-6.26℃,雌螺过冷却点显著低于雄螺;进入冬季后,福寿螺自由水含量明显下降,结合水、甘油、脂肪、糖原含量均出现明显上升,除甘油外,其余各耐寒性生理指标均存在极显著的性别差异;未经过冷驯化的雌雄福寿螺经过10℃处理7d后存活率均在90%以上,继续在5℃下培养7 d后存活率出现明显的性别差异和壳高差异.野外调查发现在福寿螺自然种群中雌螺多于雄螺.表明福寿螺雌螺的耐寒能力强于雄螺.这对进一步探索福寿螺的生态适应性、自然性比及其耐寒性机制有一定的参考价值.
In order to understand whether there is gender difference in cold tolerance of the snails, the male and female snails were investigated in South China and the survival rate under low temperature stress was investigated, and their physiological and biochemical substances in cold tolerance were measured before and after entering the winter.The results showed that male and female The mean value of subcooling point was -6.83 ℃ and -6.26 ℃, respectively, and the supercooling point of female snails was significantly lower than that of male snails. After entering the winter, the free water content decreased significantly, and the content of water, glycerol, fat and glycogen increased obviously , Except for glycerol, there were extremely significant gender differences in physiological indices of cold tolerance. The surviving rates of non-cold-acclimated male and female survived more than 90% after 7 days of treatment at 10 ℃, and continued to survive at 5 ℃ for 7 days There were significant differences in sex and shell height.Field survey found that female snails were more abundant than male snails in the natural populations of B. lupulus, indicating that the cold tolerance of the snails was higher than that of male snails.This is of great value in further exploring the ecological adaptability and natural Than cold hardiness and its mechanism has some reference value.