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目的观察和测定44株不同来源阿萨希毛孢子菌(Trichosporon asahii,T.asahii)抗氧化酶活性。方法收集44株不同来源T.asahii,将其分为环境组(3株)、临床组(9株)、体内传代组(20株)和体外耐药组(12株),分别用羟胺法和可见光法测定其抗氧化酶超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性,并进行比较和分析。结果环境组:SOD酶活性均值为(4.01±0.66)U/mgprot,CAT酶活性均值为(48.51±7.60)U/mgprot;临床组:SOD酶活性均值为(10.45±3.87)U/mgprot,CAT酶活性均值为(110.56±35.77)U/mgprot;体内传代组:至传代第5代,菌株SOD酶活性均值为(8.65±4.15)U/mgprot,CAT酶活性均值为(71.36±12.19)U/mgprot;耐药组:至诱导末期第10代菌株SOD酶活性均值为(8.02±1.56)U/mgprot,CAT酶活性均值为(80.43±8.92)U/mgprot。结论 T.asahii具有较强的抗氧化能力。不同来源T.asahii抗氧化能力不同,主要体现在抗氧化酶活性的不同。其中,临床组菌株抗氧化能力最强,体内传代组菌株和体外耐药组菌株次之,环境组菌株最低。
Objective To observe and measure the antioxidant enzyme activity of 44 Trichosporon asahii (T.asahii) from different sources. Methods 44 strains of T.asahii were collected and divided into environmental group (3 strains), clinical group (9 strains), in vivo passage group (20 strains) and drug resistance group (12 strains) The activities of superoxide dismutase (SOD) and catalase (CAT) were measured by visible light method and compared. Results In the environmental group, the mean value of SOD activity was (4.01 ± 0.66) U / mgprot, and the average CAT activity was (48.51 ± 7.60) U / mgprot. In the clinical group, the average SOD activity was (10.45 ± 3.87) U / (110.56 ± 35.77) U / mgprot; In vivo passage group: The average of SOD activity was (8.65 ± 4.15) U / mgprot and the average CAT activity was (71.36 ± 12.19) U / In the drug-resistant group, the average SOD activity of the 10th generation strain was (8.02 ± 1.56) U / mgprot and the average CAT activity was (80.43 ± 8.92) U / mgprot. Conclusion T.asahii has a strong antioxidant capacity. Different sources of T.asahii antioxidant capacity is different, mainly reflected in the different antioxidant enzyme activity. Among them, the clinical strains of the strongest anti-oxidant capacity, in vivo passage group and in vitro drug-resistant strains followed, the lowest environmental group strains.