论文部分内容阅读
目的研究金属螯合剂氯碘羟喹(Clioquinol,CQ)对沙土鼠全脑缺血再灌注后海马CA3区锥体细胞的保护作用。方法无创动脉夹夹持沙土鼠双侧颈总动脉制备脑缺血模型,腹腔注射CQ 3d,应用金属自显影技术检测CQ对沙土鼠海马游离锌离子的螯合作用,TUNEL和caspase-3原位杂交分析CQ对脑缺血沙土鼠海马神经元凋亡的影响。结果脑缺血CQ处理组沙土鼠海马金属自显影阳性反应明显低于脑缺血溶剂对照组,脑缺血CQ处理组沙土鼠海马TUNEL和caspase-3阳性细胞数量明显低于脑缺血溶剂对照组。结论 CQ对沙土鼠海马游离锌离子有明显的螯合作用,可能与其对脑缺血再灌注损伤的保护作用有关。
Objective To study the protective effect of metal chelator Clioquinol (CQ) on pyramidal neurons in hippocampal CA3 area after global cerebral ischemia and reperfusion in gerbils. Methods Cerebral ischemia model was established by bilateral non-invasive dissection of carotid arteries in rabbits. CQ 3d was injected intraperitoneally. The chelation of CQ on hippocampal free zinc ions was detected by metal autoradiography. TUNEL and caspase-3 were in situ Effect of CQ on the Apoptosis of Hippocampal Neurons in Gerbils with Cerebral Ischemia. Results The positive hippocampal SMC of hippocampus in CQ group was significantly lower than that of cerebral ischemia control group. The numbers of TUNEL and caspase-3 positive cells in hippocampus of CQ group were significantly lower than those of cerebral ischemia control group group. Conclusion CQ has significant chelation effect on hippocampal free zinc ions in gerbils, which may be related to its protective effect on cerebral ischemia-reperfusion injury.