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α-干扰素(IFN-α)与高亲和膜受体结合后刺激基因表达。目前为止所有克隆出的IFN-α刺激基因的特点是都有一个DNA元件存在,该元件叫做IFN刺激应答元件(ISRE),常位于基因的5′上游区。紧随着IFN与受体结合启动的信号转换,一种或几种核因子结合到ISRE上,这为IFN-α信号的快速转换提供了一个方便的检测方法。其转换过程利用了PLA_2产生的能调节ISRE结合因子的第二信号分子。克隆表达确定了两个可能作为信号受体的特异ISRE结合蛋白。深入研究IFN作用的分子机理可以应用酵母遗传学方法,酵母中表达的人P68激酶具有生长抑制表型,从而提供了分析IFN刺激途径的成分的另一有用手段。
Alpha-interferon (IFN-alpha) stimulates gene expression upon binding to high-affinity membrane receptors. So far all cloned IFN-a stimulating genes are characterized by the presence of a DNA element called the IFN stimulation response element (ISRE), often located in the 5 ’upstream region of the gene. Immediately following the signal transduction initiated by the binding of IFN to the receptor, one or more of the nuclear factors bind to the ISRE, which provides a convenient means of detecting rapid IFN-α signaling. The conversion process utilizes a second signaling molecule produced by PLA 2 that regulates the ISRE binding factor. Clone expression identified two specific ISRE-binding proteins that may serve as signal receptors. In-depth study of the molecular mechanism of IFN action The yeast-derived human P68 kinase, with its yeast growth inhibitory phenotype, can be applied in yeast genetics and thus provides another useful means of analyzing components of the IFN-stimulatory pathway.